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التحديثات
الفهرس
التصنيفات


عنوان البحث
Glucose Deprivation Using 2-Deoxyglucose And Acarbose Induce Metabolic Oxidative Stress And Apoptosis In Female Mice Bearing Breast Cancer
عنوان المجلة
Biochimie
ISSN-2214-0085
تفاصيل النشر
سنة النشر - 2022 / الفهرس الاصلي للمجلة - 195 : 1 (عدد الصفحات 7)
تصنيف البحث
Biochemistry and physiology - المجموعة الطبية
رابط DOI
https://doi.org/10.1016/j.biochi.2022.01.007
رابط البحث
فتح في موقع المجلة الرسمي
البحث والاستدامة
غير مرتبط باهداف التنمية المستدامة  
البحث والمجتمع
نعم , يدعم
الكلمات المفتاحية
Glucose deprivation, Glycolysis inhibition, Combination therapy, Oxidative stress Apoptosis, Anti-cancer therapy

اسم الباحثجهة الانتساببلد الباحث
Qayssar Abdul Hussein Obaid University of Sumer Iraq
Khalisa Khudair University of Baghdad Iraq
Ahmed Majeed Alshammari University of Baghdad Iraq

A characteristic of cancer cells is increased glucose uptake and glycolysis for energy production and hydroperoxide detoxification due to mitochondrial dysfunction. Thus, inhibition of glucose uptake and glycolysis represent smart novel therapy. We used 2-deoxyglucose (2DG) as a glycolysis inhibitor and acarbose (ACA), a specific alpha-glucosidase inhibitor, to decrease glucose uptake. Mice bearing mammary adenocarcinoma tumors were treated by 2DG and/or ACA. Relative tumor volume, tumor growth inhibition rate, relative body weight, glucose concentration, hexokinase-1 protein level by ELISA, pyruvate, and ATP (glycolysis products), reactive oxygen species (ROS), total glutathione T-GSH, apoptosis, and histopathology were measured in treated and untreated groups. Our results showed that combination therapy inhibited tumor volume and increased tumor growth inhibition rate, body weight reduction, decreasing glucose level, HK-1 level, and inhibition of glycolysis products. In addition, combination therapy induced oxidative stress, increase ROS, and decrease T-GSH. Furthermore, immunohistochemistry examination showed the broader area of apoptosis in breast cancer treated by combination agents. In conclusion, our result revealed that the novel combination inhibits glycolysis and glucose uptake and induced oxidative stress and apoptosis.