عرض تفاصيل البحث

ادناه جميع التفاصيل الخاصة بالبحث المطلوب عرضه. توفر هذه المنصة معلومات اساسية حول البحث, لمزيد من التفاصيل يرجى متابعة التصفح من خلال الضغط على الرابط الاصلي للبحث او رابط DOI.

التحديثات
الفهرس
التصنيفات


عنوان البحث
Molecular Characterization Of Antigens Extracted From Hydatid Cysts Of Human And Other Intermediate Hosts
عنوان المجلة
Global Science Research Journals
ISSN-: 2449-1888
تفاصيل النشر
سنة النشر - 2010 / الفهرس الاصلي للمجلة - 7 : 2 (عدد الصفحات 7)
تصنيف البحث
Microbiology - المجموعة الطبية
رابط DOI
#
رابط البحث
فتح في موقع المجلة الرسمي
البحث والاستدامة
غير مرتبط باهداف التنمية المستدامة  
البحث والمجتمع
نعم , يدعم
الكلمات المفتاحية
Hydatidosis, Echinococcus granulosus, polymerase chain reaction (PCR)

اسم الباحثجهة الانتساببلد الباحث
Mohammed Musa Ata University of Sumer Iraq
Maani Alshemari University of Qadysia Iraq.
Ihsan Alsaimary University of Basrah Iraq

This research is a study on the molecular level of Echinococcus granulosus (larval stage) on, where 7 samples of hydatid cysts were collected from parasite intermediate hosts: Human (liver, spleen, lung) and liver of sheep, goat, cattle and buffaloes. DNA was extracted from germinal layer cells of hydatid cysts which were isolated shortly or preserved for various periods in 70% ethanol. Genetic analysis of isolated DNA from hydatid cysts collected from human and animals was done by polymerase chain reaction (PCR) to determine genetic variation depending on random amplified polymorphic DNA. In the present study, 10 primers were used during which the genetic variations were revealed among isolated (extracted DNA) hydatid cysts which was collected from human and other intermediate hoists except cows and buffaloes. The results of this study showed the following: 1. One primer (OPA – 01) was able to diagnose sample 1 which represent the isolated DNA of liver hydatid cyst obtained from human at age group 10 – 20 years old: 2. The ability of primer OPC – 10 to determine fingerprinting of DNA sample of sheep liver hydatid cyst. 3. The ability of primer OPC – 05 to determine fingerprinting of DNA sample of human spleen hydatid cyst which was obtained from human at age group 30 – 40 years old. The ability of primer OPE – 07 to determine fingerprinting of DNA sample of Goat liver hydatid cyst; 5. Amplification process to the DNA samples extracted from cows and buffaloes liver hydatid cysts was not completed by using all 10 primers.